4E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 bind strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis.
Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Western Blot: 1:5,000-10,000
Immunohistochemistry (paraffin): 1:50-100
Immunocytochemistry: 1:100-250
Immunoprecipitation: 1:10
Optimal dilutions to be determined by the researcher.
Positive Control:
Untreated and FBS-treated 293T cell lysate.
Storage and Stability:
May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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