Home  >  Products  >  4E Binding Protein 1, phosphorylated (Thr37) (4EBP1, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)
4E Binding Protein 1, phosphorylated (Thr37) (4EBP1, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

4E Binding Protein 1, phosphorylated (Thr37) (4EBP1, 4E-BP1, Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Cat no: 0004-19H

Supplier: United States Biological
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4E-BP1 is a eukaryotic translation initiation factor (eIF4E- binding protein), also known as PHAS-1. The binding of 4E-BP to eIF4E is regulated by phosphorylation and is reversible. Non-phosphorylated 4E-BP1 bind strongly to eIF4E; however, phosphorylated 4E-BP1 will not bind to eIF4E. Akt, TOR, MAP, S6, and Cdc2 kinases are able to inactivate 4E-BP1 by phosphorylation. 4E-BP1 undergoes phosphorylation which releases it from eIF4E, ultimately forming initiation complexes that aid in the upregulation of protein synthesis. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1,000-2,000 Optimal dilutions to be determined by the researcher. Positive Control: Untreated and insulin-treated 293T cell lysate. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: 0004-19H
Reactivities: Human, Mouse
Hosts: Rabbit
Applications: Western Blot
Size: 100ul
Form: Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
P type: Mab
Isotype: IgG
Purity: Supernatant
References: 1. Pause, A., et al. (1994). Nature 371: 762-767. 2. Gingras, A.C., et al. (1998). Genes & Dev. 12: 502-513. 3. Iritani, B.M., et al. (1999). Proc. Natl. Acad. Sci. 96, 13180-13185.
Additional info: Recognizes human 4E-BP1 phosphorylated at Thr37. Species Crossreactivity: mouse.

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