4E Binding Protein 1 (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

4E-BP1(eukaryotic translation Initiation Factor 4E Binding Protein 1), also called ELF4EBP1/BP-1/PHAS-I , is located on chromosome 8p12, with 118-amino acid protein (about 13kD). Binding of eIF4EBP1 to eIF4E is reversible and is dependent on the phosphorylation status of eIF4EBP1. Non-phosphorylated eIF4EBP1 will bind strongly to eIF4E while the 24kD phosphorylated form will not. Akt, TOR, MAP kinase, S6 kinase, and Cdc2 are known kinases capable of inactivating eIF4EBP1 binding to eIF4E by phosphorylating either threonines 35, 45, 69 or serine 64. Although, not all phosphorylation events equally block the eIF4EBP1-eIF4E interaction.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:10,000\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

0004-22C

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid, 0.03% sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Pause, A. et al. 1994.Nature. 371:762-767. \n2. Fadden, P. et al. 1997. J. Biol. Chem. 272:10240-10247.

Additional Info

Recognizes human 4E Binding Protein 1.

Alternative Names

Eukaryotic Translation Initiation Factor 4E Binding Protein 1, 4E-BP1, 4EBP1, BP-1, BP1, MGC4316, PHAS I, PHASI, Phosphorylated Heat and Acid Stable Protein Regulated by Insulin 1, eIF4E Binding Protein 1, eIF4EBP1