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5-Fluoroorotic Acid Monohydrate (FOA, 5-FOA)

Cat no: F5050

5-Fluoroorotic Acid Monohydrate (FOA, 5-FOA)

Useful in the identification and selection of the strains of Saccharomyces cerevisiae that contain the mutant ura3- gene. 5-FOA is toxic to yeast cells that can synthesize the enzyme orotidine-5

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SPECIFICATIONS

Catalog Number

F5050

Size

500mg

Form

Off-white to light yellow powder

Purity

(same/more than)98%

References

US Biological application references: 1. Schmidt, H. K. et al., (2006) Meth Enzymology 409:462-476. 2. Cottingham, F. R. and Hoyt, M. A. (1997) J. Cell Biology 138:1041-1053. 3. Luca, F. C. and Winey, M. (1998) Mol. Biol. Cell 9:29-46. 4. Dang, V.-D. and Levin, H.L. (2000) Mol. Cell Biol. 20:7798-7812. 5. Teysset, L. et al., (2003) J. Virology 77:5451-5463. 6. Dang, V. D. et al., (1999) Mol. Cell. Biol. 19:2351-2365. 7. Atwood-Moore, A. et al., (2005) J. Virology 79:14863-14875. 8. Liu, Y. and Bambara, R.A. (2003) J. Biol. Chem. 278:13728-13739. 9. Maldonado-Baez, L. et al., (2008) Mol. Biol. Cell 19:2936-2948. 10. Wu, H. et al., (2010) Mol. Biol. Cell 21:430-442. 11. Munox-Centeno, M. et al., (1999) Mol. Biol. Cell 10:2393-2406. 12. McClelland, C. M. et al., (2002) Genetics 160:935-947. 13. Tsubouchi, H. and Ogawa, H. (1998) Mol. Cell. Biol. 18:260-268. 14. Brunson, L.E., et al., (2005) Molecular Genetics and Genomics 273:361-370. 15. Xia, X, et al., (2002) Mutation Research 519:83-92. 16. Jia, X. and Xiao, W. (2003) Toxicological Sciences 75:82-88. 17. Jacquiau, H.R. et al., (2005) J. Biol. Chem. 280:23566-23575. 18. Kozminski, K.G. et al., (2000) Mol. Biol. Cell 11:339-354. 19. Tokarev, A.A. et al., (2009) Traffic 10:1831-1844. 20. Si, H. et al., (2010) Genetics 110:114165. 21. Bultynck, G. et al., (2006) Mol. Cell. Biol. 26:4729-4745. 22. Leu, J.Y. and Murray, A. W. (2006) Curr Biol. 16:280-286. 23. Jia, X. et al., (2002) Mutat Res. 519:83-92. 24. Pruyne, D.W. et al., (1998) J Cell Biol. 143:1931-45. 25. Yasutis, K. et al., (2010) Mol. Biol. Cell 21:4373-4386. 26. Manlandro, C.M.A. et al., (2012) FEMS Yeast Research DOI: 10.1111/j.1567-1364.2012.00814.x. 25. Chung, D. , et al., PLoS One (2012) 7; e43844. doi:10.1371/journal.pone.0043844. 1. Winston, F., et al., Genetics 107: 179 (1984). 2. Boeke, J., et al., Mol. Gen. Genetics 197: 345 (1984). 3. Hinnen, A., et al., PNAS USA 75: 1929 (1978). 4. Ito, H.,et al., J. Bacteriol. 153: 163 (1983). 5. Mann, C., et al., Cell 48: 627 (1987). 6. Ausubel, F.M., et al., Current Protocols in Molecular Biology, John Wiley (1992). 7. Maniatis, T., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press (1989). 8. Bartel, P.L., Fields, S., Yeast 2-Hybrid System, Oxford University Press, New York, 7: 109-147 (1997).

Alternative Names

5-Fluoroorotate; 5-FLUORO-4-PYRIMIDINECARBOXYLIC ACID; 5-FLUORO-1,2,3,6-TETRAHYDRO-2,6-DIOXO-(9CI); 1,2,3,6-TETRAHYDRO-2,6-DIOXO-5-FLUORO-4- PYRIMIDINECARBOXYLIC ACID

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Hum

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ELISA, ICC, IHC, WB

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Rat

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Applications

ELISA, ICC, IHC, WB

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Rabbit

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Hum

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Applications

ELISA, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Mouse

More info

Applications

ELISA, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Rat

More info

Applications

ELISA, ICC, IHC, WB

Hosts

Rabbit

Reactivities

Mouse

More info
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