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5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine)

5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine)

Cat no: 045666

Supplier: United States Biological
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5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming. 5-Hydroxymethylcytosine is a DNA pyrimidine nitrogen base formed from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of iron-dependent oxygenases. Data suggests that every mammilian cell contains 5-hydroxymethylcytosine, but the levels vary depending on the cell type; data also suggests that levels of 5-hydroxymethylcytosine increases with age. The highest levels are found in neuronal cells of the central nervous system and certain mammalian tissues such as mouse Purkinje and granule neurons. Although the exact function has not been fully elucidated, studies suggest that 5-hydroxymethlcytosine may regulate gene expression or initiate DNA demethylation. Applications: Suitable for use in Dot Blot Recommended Dilution: Dot Blot: 10ug/ml and 1ug/ml detected 5-hydroxymethylcytosine in a panel of unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine DNA. Control: Unmodified DNA, 5-Methylcytosine DNA, and 5-hydroxymethylcytosine Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: 045666
Hosts: Rat
Applications: Immunocytochemistry
Size: 100ug
Form: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.05% sodium azide.
P type: Mab
Isotype: IgG2a,k
Purity: Purified by Protein G affinity chromatography.
References: Wossidlo, Mark, et al. (2011) Nat Commun, 2: 241 (2011)
Additional info: Detects 5-hydroxymethyl cytosine, but not 5-methylcytosine or unmethylated cytosine. Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.

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