5-Hydroxymethyluridine (5-HmdU)

5-Hydroxymethyl-2'-deoxyuridine (5-HmdU) is formed from thymidine during the oxidation of DNA by ionizing radiation or reactive oxygen species (ROS) that are formed from inflammatory response. This causes genetic mutations, altered gene expression and chromosomal instability that have generally been implicated with aging, cancers and autoimmune diseases.\n\nSystemic oxidative stress levels, as evidenced by DNA damage products in the blood and urine, appear to be associated with a risk of various cancers, including that of the breast, lung and colorectal cancers. Cigarette smoke contains numerous chemical carcinogens and other compounds that generate reactive oxygen species that can damage DNA directly or indirectly via inflammatory processes. 5-HmdU is one of the major oxidized DNA bases that is significantly elevated in the white blood cells of women diagnosed with breast cancer, which may be a result of diminished DNA repair. Individuals with BRCA1 and BRCA2 mutations may be at increased risk for cancer due to deficiencies in the repair of DNA lesions caused by ROS. Additionally, antibody titers recognizing 5-HmdU have also been found to be significantly elevated in women with breast, color and rectal cancers. Therefore, oxidative DNA damage is therefore an attractive marker of disease risk as it takes into account not only the exposure to and production of oxidants, but also the cells' DNA repair ability.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136858

Size

100ul

Applications

WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Lis, A.W., Mclaughlin, R.K. and Mclaughlin, D.I. Studies on 5-hydroxyuridine (isobarbituridine): X. Isolation and tentative identification of 5-hydroxymethyluridine from ribosomal RNA. Physiol Chem Phys. 1975;7(6):565-70.

Additional Info

Recognizes human 5-HmdU.