5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit

Cat no: ABIN1873480

Supplier: Antibodies-online

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5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to 5-Me-THF has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled 5-Me-THF and unlabeled 5-Me-THF (Standards or samples) with the pre-coated antibody specific to 5-Me-THF. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of 5-Me-THF in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of 5-Me-THF in the sample.

Catalogue number:	ABIN1873480
Size:	96 tests
Antigen:	5-Methyltetrahydrofolate (5-Me-THF)
Sensitivity:	The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
Assay range:	1.11-90 ng/mL
Sample type:	Serum,Plasma,Biological Fluids

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5-Methyltetrahydrofolate (5-Me-THF) ELISA Kit

Cat no: ABIN1873480

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