5'-Nucleotidase (CD73, CD73 Antigen, Ecto-5'nucleotidase, 5'-NT, eN, eNT, NT5, NT5E, NTE, Purine 5 Prime Nucleotidase)

CD73 (Ecto-5'nucleotidase, 5?'-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:500-1:1000\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD73 protein. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

N7100-71A

Size

50ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2b,k

References

1.Sern P.C, et al., (2006) Purinergic Signal 2(2):351-360 2. Linda F.T, et al., (2004) J Exp Med 200(11):1395-1405 3. Laura A, et al., (1997) J Cell Biol 136(2):421-431

Additional Info

Recognizes human 5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT).