logo
logo
5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT)

Cat no: N7100-71B

5'-Nucleotidase (CD73, Ecto-5'nucleotidase, 5'-NT)

CD73 (Ecto-5'nucleotidase, 5?'-NT) is a membrane-bound glycoprotein that functions to hydrolyze extracellular nucleoside monophosphates into bioactive nucleoside intermediates. This enables the uptake of adenosine, inosine, and guanosine into the cell and their subsequent reconversion into ATP and GTP in the purine salvage pathway. The expression of CD73 on neuronal cells has been described during developmental processes and, on lymphocytes, CD73 serves as a maturation marker, being absent from the surface of both immature B and T cells.\n\nApplications: \nSuitable for use in Direct ELISA, Western Blot. and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nDirect ELISA: 0.5-1.0ug/ml .Detection limit for rmCD73 is \n~0.3ng/well.\nWestern Blot: 0.1-0.2ug/ml. Detection limit for rmCD73 is ~1ng/lane under non-reducing and reducing conditions.\nFlow Cytometry (Murine splenocytes): 25ug/ml. Add 100ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not excedding 200ul.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, PBS. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

N7100-71B

Size

100ug

Applications

ELISA, FC, WB

Hosts

Sheep

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile 40-50% glycerol, PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.\n

Isotype

IgG

Additional Info

Recognizes mouse CD73.

Read more on Supplier website
Advertisehere
MtoZ Horizontal banner
MtoZ Biolabs

Latest promotion FROM:

MtoZ Biolabs
View promotion

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...