8-Hydroxydeoxyguanosine (8OHdG)

8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease.\n\n8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress.\n\nApplications:\nSuitable for use in Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry: 1:200\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136859

Size

100ul

Applications

IHC

Hosts

Goat

Reactivities

Hum, Mouse, Rat, Bov, Fel, Prc

Form

Supplied as a liquid, 0.09% sodium azide.

P Type

Pab

Purity

Serum

Isotype

IgG

References

1. Murata, T., Ohtsuka, C. and Terayama, Y. Increased mitochondrial oxidative damage and oxidative DNA damage contributes to the neurodegenerative process in sporadic amyotrophic lateral sclerosis. Free Radic Res. 2008 Mar;42(3):221-5. 2. Tanuma, N., Miyata, R., Hayashi, M., Uchiyama, A. and Kurata, K. Oxidative stress as a biomarker of respiratory disturbance in patients with severe motor and intellectual disabilities. Brain Dev. 2008 Jun;30(6):402-

Additional Info

Recognizes 8OHdG. Species Crossreactivity: human, rat, mouse, primate, porcine, bovine and feline.