8-Oxoguanine (8-Hydroxyguanine)

8-Oxoguanine is a mutagenic oxidative damage product of guanine. Oxidatively damaged base 8-oxoguanine is generated in the DNA of all living organisms due to the presence of reactive oxygen species in cells. DNA damage threatens the health of the genetic information stored in every DNA molecule; however enzymes exist in cells to protect against the mutagenic effect of this lesion. 8-oxoguanine is one of the most abundant and well-characterized DNA lesions generated by oxidative stress. It has been estimated that ~180 guanines are oxidized to 8-oxoG per mammalian cell per day. 8-oxoG is a miscoding lesion that can cause G:C to T:A or T:A to G:C transversion mutations. This lesion accumulates in DNA with age and it has been loosley linked to several cancers and diseases such as Alzheimer's and Parkinson's.\n\nApplications: \nSuitable for use in ELISA and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunocytochemistry: Detects 8-Oxoguanine in HeLa and Cos7 cells fixed with paraformaldehyde. 8-Oxoguanine has been localized to the nucleus in nutrient-deprived cells.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control:\nNutrient starved HeLa cells or Cos7 cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

O8235

Size

100ul

Applications

ELISA, ICC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, NHP

Form

Supplied as a liquid. No preservative added.

P Type

Mab

Purity

Ascites

Isotype

IgM

References

1. Conlon, K., et al., J. Histotechnology 23: 37-44 (2000).

Additional Info

Recognizes 8-oxoguanine. Exhibits reactivity with dGMP, dAMP, dCMP or TMP in millimolar, but not in micromolar concentrations. Species Crossreactivity: human, monkey, rat and mouse.