a-Actin, Smooth Muscle

Among the six actin isoforms described in mammals, two are found in virtually all cells (beta- and gamma-cytoplasmic), two are detected in smooth muscle cells (alpha- and gamma-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (a-skeletal) and one in cardiac (a-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus and the sequences of each of them are perfectly conserved in higher vertebrates. Alpha-smooth muscle actin is abundant in vascular and visceral smooth muscle cells. In addition, it has also been shown to appear in stress fibers of fibroblastic cells during pathological situations involving contractile phenomena such as wound healing and fibrocontractive diseases.\n\nApplications:\nSuitable for use in ELISA, Western Blot, Immunohistochemistry and Immuno-electron Microscopy. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:10,000-1:50,000\nImmunohistochemistry (Paraffin): 1:10-1:100\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nDerived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BALB/c mouse.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0760-29A

Size

50ul

Applications

ELISA, IHC, WB

Hosts

Mouse

Reactivities

Hum, Rat, Ch/Bird

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

P Type

Mab

Purity

Purified

Isotype

IgG2a

References

1. Skalli, O., et al., J. Cell. Biol. 103: 2787-2796 (1986). 2. Skalli, O., et al., Lab. Invest. 60: 275-285 (1989). 3. Babaev, V.R., et al., Am. J. Pathol. 136: 1031-1042 (1990) 4. Sappino, A.P., et al., Lab. Invest. 63: 144-161 (1990). 5. Vyalov, S.L., et al., Am. J. Pathol. 143: 1754-1765 (1993). 6. Chaponnier, C., et al., J. Cell. Biol. 130: 887-895 (1995). 7. Simoncelli, F., et al., Eur. J. Cell Biol. 69: 297-300 (1996). 8. Hinz, B., et al., Mol. Biol. Cell 12: 2730-2741 (2001). 9. Hinz, B., et al., J. Cell. Biol. 157: 657-663 (2002). 10. Hinz, B., et al., Mol. Biol. Cell 14: 2508-2519 (2003). 11. Clement, S., et al., J. Cell Sci. 118: 1395-1404 (2004). 12. Chaponnier, C. & Gabbiani, G., J. Pathol. 204: 386-395 (2004). 13. Clement, S., et al., J. Cell Sci. 120: 229-238 (2006). 14. De Visscher, G., et al., Mol. Morphol. 18: 382-391 (2010).

Additional Info

Recognizes exclusively alpha-smooth muscle actin which is typical for vascular and visceral smooth muscle cells, but which is also present in myofibroblasts. The epitope that is recognized by a-SM1 is Ac-EEED.