a-Catenin

Adherens junctions (AJ) (also referred to as zonula adherens) are required for both the establishment and maintenance of epithelial layers (2). In addition, these junctions have been identi?ed in several other cell types including cardiac myocytes and ?broblasts (2). AJ subserve several important functions including: mediating intercellular adhesion, sensing the presence of neighboring cells, and anchoring the actin cytoskeleton (2,3). AJ are multiprotein complexes that are assembled around cell adhesion molecules called cadherins. Cadherins are a multifunctional family of Ca2+-dependent, transmembrane, glycoproteins which promote cell-cell adhesion (4). The cadherin extracellular domain mediates homophilic interactions between like cadherin molecules on neighboring cells, while the intracellular domain interacts with several cytoplasmic proteins which include: a-catenin, b-catenin, g-catenin (plakoglobin), and the tyrosine kinase substrate p120 cas (1,5,6,7,8,9). Cadherin-catenin interactions are required for complete cadherin activity and regulate the interaction between cadherins and the actin-based cytoskeleton (1,4,6,8,9,10). In fact, deletion of the cadherin cytoplasmic domain produces an adhesion-defective molecule that is unable to interact with the cytoskeleton (4,11).\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.1-1ug/ml\nWestern Blot: 1-3ug/ml\nImmunoprecipitation: 5-15ug \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

C2069-44

Size

100ug

Applications

ELISA, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat, Ch/Bird, Xen/Amph

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Herrenknecht, K., et al., Proc. Natl. Acad. Sci. USA 88: 9156-9160 (1991). 2. Geiger, B., et al., Curr. Opin. Cell Biol. 1: 103-109 (1989). 3. Peifer, M., et al., Science 262: 1667-1668 (1993). 4. Takeichi, M., et al., Science 51: 1451-1455 (1991). 5. Daniel, J.M., et al., Mol. Cell. Biol. 270: 4819-4824 (1995). 6. Hinck, L, et al., J. Cell. Biol. 125: 1327-1340 (1994). 7. Knudsen, K.A., et al., J. Cell. Biol. 18: 671-679 (1992). 8. McCrea, P.D., et al., J. Biol. Chem. 266: 4514-4520 (1991). 9. Ozawa, M., et al., Proc. Natl Acad. Sci. USA. 87: 4246-4250 (1990). 10. Knudsen, K.A., et al., J. Biol. Chem. 130: 67-77 (1995). 11. Ozawa, M., et al., J. Cell. Biol. 116: 989-996 (1992). 12. Nagafuchi, A., et al., Cell 65: 849-857 (1991). 13. Nagafuchi, A., et al., J. Cell Biol. 127: 235-245 (1994). 14. McGregor, et al., Biochem. J. 301: 225-233 (1994). 15. Menkel, A.M., et al., J. Cell Biol. 126: 1231-1240 (1994). 16. Rimm, D.L., et al., Proc. Natl. Acad. Sci. USA 92: 8813-8817 (1995). 17. St. Croix, B., et al., J. Cell Biol. 142(2): 557-571 (1998).

Additional Info

Speci?c for a-catenin, a 102kD protein. Does not crossreact with the related b-catenin or g-catenin proteins. \nSpecies Crossreactivity: Human, Mouse, Rat, Chicken, Xenopus. Tissues/Lysates Tested: HeLa cells, A431 cells, WI-38 human ?broblasts, Xenopus Cytosol, NIH 3T3 cells.