a-Fetoprotein (AFP)

AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested.\n\nRecommended Dilution:\nImmunohistochemistry (paraffin-embedded sections): 1:200 using ABC method.\nWestern Blot: 2-4ug/ml\nOptimal dilutions to be determined by the researcher.\n\nCellular Localization: Secreted, Cytoplasmic\n\nPositive Control: human fetal liver protein\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4100-03B

Size

500ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid containing sodium azide

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

References

1. Jacobsen GK et al. Distribution of tumor-associated antigens in the various histologic components of germ cell tumors of the testis. Am J Surg Pathol 5:257-66 (1981). 2. MacDonald DJ & Kelly AM. The rapid quantitation of serum alpha-fetoprotein by two-site micro enzyme immunoassay. Clin Chim Acta 87:367-72 (1978).

Additional Info

Recognizes human alpha-Fetoprotein (AFP)

Alternative Names

Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP