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a-Fetoprotein (AFP)

a-Fetoprotein (AFP)

Cat no: F4100-03B

Supplier: United States Biological
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AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. Applications: Suitable for use in Western Blot and Immunohistochemistry (paraffin-embedded sections). Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin-embedded sections): 1:200 using ABC method. Western Blot: 2-4ug/ml Optimal dilutions to be determined by the researcher. Cellular Localization: Secreted, Cytoplasmic Positive Control: human fetal liver protein Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: F4100-03B
Reactivities: Human
Hosts: Rabbit
Applications: Immunohistochemistry, Western Blot
Size: 500ul
Form: Supplied as a liquid containing sodium azide
P type: Pab
Isotype: IgG
Purity: Purified by immunoaffinity chromatography
Alternative names: Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP
References: 1. Jacobsen GK et al. Distribution of tumor-associated antigens in the various histologic components of germ cell tumors of the testis. Am J Surg Pathol 5:257-66 (1981). 2. MacDonald DJ & Kelly AM. The rapid quantitation of serum alpha-fetoprotein by two-site micro enzyme immunoassay. Clin Chim Acta 87:367-72 (1978).
Additional info: Recognizes human alpha-Fetoprotein (AFP)

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