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a-Fetoprotein (Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP )

Cat no: F4100-01K

a-Fetoprotein (Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP )

AFP (alpha-Fetoprotein) is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. Alpha-Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature human AFP is 591 amino acids (aa) in length. It contains three albumin domains (aa 19-210, 211-402 and 403-601), plus 15 intrachain disulfide bonds. Mature human AFP shares approximately 66% aa identity with mouse AFP.\n\nApplications: \nSuitable for use in Direct ELISA, Flow Cytometry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunocytochemistry: 8-25ug/ml using Immersion fixed HepG2 human hepatocellular carcinoma cell line\nFlow Cytometry: 2.5ug/10e6 cells using HepG2 human hepatocellular carcinoma cell line fixed with paraformalydehyde and permeabilized with saponin.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

F4100-01K

Size

100ug

Applications

ELISA, FC, ICC

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Matsumura, M. et al., 2001, Hepatol. Res. 20(1):84 - 96. 2. Deutsch, H.F. et al., 2000, Tumor Biol. 21(5):267 - 277.

Additional Info

Recognizes human alpha-Fetoprotein.

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