AFP (alpha-Fetoprotein) is a 69-73 kDa member of the ALB/AFP/VDB family of proteins. Alpha-Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature human AFP is 591 amino acids (aa) in length. It contains three albumin domains (aa 19-210, 211-402 and 403-601), plus 15 intrachain disulfide bonds. Mature human AFP shares approximately 66% aa identity with mouse AFP.
Applications:
Suitable for use in Direct ELISA, Flow Cytometry and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Immunocytochemistry: 8-25ug/ml using Immersion fixed HepG2 human hepatocellular carcinoma cell line
Flow Cytometry: 2.5ug/10e6 cells using HepG2 human hepatocellular carcinoma cell line fixed with paraformalydehyde and permeabilized with saponin.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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