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a-Fetoprotein (Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP)

Cat no: F4100-05T

a-Fetoprotein (Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP)

Alpha-fetoprotein (AFP) is a 65kD glycoprotein found in the serum of the mammalian fetal liver, yolk sac and GI tract. Expression of AFP in adult cells is low. However, synthesis aberrantly occurs in adult liver cancer cells (1,2). The tumor suppressor gene p53 and beta-catenin are both involved in the regulation of AFP expression. In normal adult cells p53 binds to the repressor region of the AFP gene, thereby blocking transcription. Mutations in both p53 and beta-catenin are associated with aberrant expression of AFP. Elevated serum levels are predictive of hepatocellular carcinoma (3).\n\nApplications:\nSuitable for use in Immunofluorescence (IF-IC) and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:100\nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

F4100-05T

Size

100ul

Applications

ELISA, IF

Hosts

Mouse

Reactivities

Hum, Mouse

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

P Type

Mab

Purity

Ascites

Isotype

IgG2A

Additional Info

Recognizes endogenous levels of total AFP protein. Species crossreactivity: Mouse.

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