AFP (a-Fetoprotein) is a 69-73kD member of the ALB/AFP/VDB family of proteins. It is secreted by fetal liver and serves as a carrier molecule for phytoestrogens, heavy metals (Cu and Ni), estrogen and fatty acids. Mature mouse AFP is 587 amino acids in length. It contains three albumin domains (aa20-201, 208-393 and 400-591), plus 15 intrachain disulfide bonds. Rat AFP contains an alternate start site at Met287 that may generate an intracellular 37kD form. Mouse AFP also shows an analogous site at Met281 that may produce an equivalent isoform. Over aa19-605, mouse AFP shares 83% and 66% aa identity with rat and human AFP, respectively.
Applications:
Suitable for use in Direct ELISA and Western Blot. Other applications not tested.
Recommended Dilutions:
Western Blot: 1ug/ml; Tested on mouse plasma and rat placenta.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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