Home  >  Products  >  a-Fetoprotein (Alpha Fetoprotein, Alpha-fetoprotein Precursor, AFP, Alpha-1-fetoprotein, Alpha-fetoglobulin, FETA, HPAFP)
a-Fetoprotein (Alpha Fetoprotein, Alpha-fetoprotein Precursor, AFP, Alpha-1-fetoprotein, Alpha-fetoglobulin, FETA, HPAFP)

a-Fetoprotein (Alpha Fetoprotein, Alpha-fetoprotein Precursor, AFP, Alpha-1-fetoprotein, Alpha-fetoglobulin, FETA, HPAFP)

Cat no: F4100-02E

Supplier: United States Biological
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D10 has been characterized in the ISOBM TD-2 workshop and assigned by K. Nustad to group D of a cluster of 6 major epitopes of human alpha fetoprotein. Its gene has been cloned and sequenced and its structure identified. AFP is expressed in fetal liver and is normally absent in healthy adult tissues. It is expressed in all yolk sac tumors, in some germ cell tumors and in hepatocellular carcinomas. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen, formalin fixed paraffin sections Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: F4100-02E
Reactivities: Human
Hosts: Mouse
Applications: ELISA, Immunohistochemistry, Western Blot
Size: 100ug
Form: Supplied as a liquid in PBS, 0.05% sodium azide.
P type: Mab
Isotype: IgG1
Purity: Purified
References: 1. Tsung K., Milunsky A., Alpert E. Derivation and characterization of a monoclonal hybridoma antibody specific for human alpha-fetoprotein. J. Immunol. Methods 39: 363-368, (1980) 2. Michell B., Fiebach H., Karsten U., Goussev A.I., Yazova A.K., Knopp J. Monoclonal antibodies to different epitopes of human alpha-fetoprotein (AFP). Eur. J. Cancer Clin. Oncol. 19:1239-1246, (1983). 3. Yazova A.K., Goussev A.I., Poltorania V.S., Yakimenko E.F., Human alpha- fetoprotein epitopes as revealed by monoclonal antibodies. Immunol. Lett. 25: 325-330, (1990). 4. Nustad K., Paus E., Kierulf B., Bormer O.P. Specificity and affinity of 30 monoclonal antibodies against alpha-fetoprotein. Tumor Biol 19: 293-300, (1998). 5. Tumor Biol 19: 301-309, (1998).
Additional info: Recognizes human alpha fetoprotein at ~70kD. Does not react with mouse, rat, canine or calf.

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