The kappa immunoglobulin light chain comprises ~60% of light chains in humans. Immunohistochemical detection of immunoglobulins is useful in determining the lymphoid origin of tissues and in the assessment of lymphoid proliferations as reactive or malignant. The assessment of light chain status is particularly important in this regard as it may be used to demonstrate the clonal nature of a tumor.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (Frozen, Paraffin): 1:10-1:40. Requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Optimal dilutions to be determined by the researcher.
Positive Control:
Placenta
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.