Alpha-fetoprotein is a 67kD tumor-associated embryonic molecule, present in mammalian embryonic and serum synthesized by the fetal liver and yolk sac. The gene encoding the protein maps to 4q11-q13 region of human chromosome. The mature protein consists of 590aa and 15 regularly spaced disulfide bridges, which generate a folding structure having three repeating domains. Alpha-fetoprotein is usually found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The protein is thought to be the fetal counterpart of serum albumin. Being a bifunctional protein it acts in cellular defence against oxidative stress both as a copper buffer and as a superoxide radical scavenger.
Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.
Recommended Dilution:
Immunohistochemistry (formalin fixed paraffin embedded): 1:50-1:150
Optimal dilutions to be determined by the researcher.
Positive Control:
Fetal liver
Storage and Stability:
May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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