One of the best studied is the alpha-foetoprotein. It cab easily be identified immunochemically
because of its distinct antigenicity. In electrophoresis its relative mobility is between the albumin and alpha-1 globulin fractions. Its molecular weight is 64000, the carbohydrate content is relatively low. The concentration of alpha-foetoprotein gradually decreases during foetal growth. It is not found in normal adult serum, but reappears in most human developing hepatoma.
Applications:
Suitable for use in ELISA, Immunoelectrophoresis and Single and Double Radial Immunodiffusion. Other applications not tested.
Recommended Dilution:
Immunoelectrophoresis: 2ml plasma or equivalent against 120ml antiserum
Double Radial Immunodiffusion: Use a rosette arrangement with 10ml antiserum in 3mm
diameter center well and 2ml plasma samples (neat and serially diluted) in 2mm diameter
peripheral wells.
Single Radial Immunodiffusion: 1% in the gel.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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