a-Fetoprotein, Human, IgG (AFP) BioAssay(TM) ELISA Kit

The United States Biological AFP Enzyme Immunoassay test kit is intended for the quantitative determination of AFP concentration in human serum. \n\nAlpha-fetoprotein (AFP) is a glycoprotein with a molecular weigh of approximately 70,000 daltons. AFP is normally produced during fetal and neonatal development by the liver, yolksac, and in small concentrations by the gastrointestinal tract. After birth, serum AFP concentrations decrease rapidly, and by the second year of life and thereafter only trace amounts are normally detected in serum. Elevation of serum AFP to abnormally high values occurs in several malignant diseases, most notably nonseminomatous testicular cancer and primary hepatocellular carcinoma. In the case of nonseminomatous testicular cancer, a direct relationship has been observed between the incidence of elevated AFP levels and the stage of disease. Elevated AFP levels have also been observed in patients diagnosed with seminoma with nonseminomatous elements, but not in patients with pure seminoma. In addition, elevated serum AFP concentrations have been measured in patients with other noncancerous diseases, including ataxia telangiectasia, hereditary tyrosinemia, neonatal hyperbilirubinemia, acute viral hepatitis, chronic active hepatitis, and cirrhosis. Elevated serum AFP concentrations are also observed in pregnant women. Therefore, AFP measurements are not recommended for use as a screening procedure to detect the presence of cancer in the general population. \n \nDetection Range: ~0-300ng/mL \n\nSample: ~20ul serum \n\nSpecificity: ~97% \n\nSensitivity: ~2.0 ng/ml \n\nTotal Time: ~80 min \n\nKit Components: \nEach kit contains the following components in sufficient quantities to perform the number of tests indicated. All reactive reagents contain 0.1% sodium azide as a preservative.\n1. Microtiter Plate\n2. Conjugate\n3. Calibrator\n4. Positive Control\n5. Negative Control\n6. TMB\n7. Wash Buffer, 10X Diluted wash buffer to 1X\n8. Sample Diluent\n9. Stop Solution \n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

F4100-11

Size

96Tests

Applications

ELISA

References

1. Abelev G I. Alpha-fetoprotein as a marker of embryo-specific differentiation in normal and human \ntissues. Transplant Rev 1974; 20:3-37. 2. Hirai H. Alpha fetoprotein. In: Chu T M, ed. Biochemical markers for cancer. New York: Marcel Dekker, 1982: 23-59. 3. Chan D W, et.al., Affinity chromatographic separatoin of alpha-fetoprotein variants: Development of a mini-column procedure and application to cancer patients. Clin Chem 1986; 32:2143-2146. 4. Sell L S. Cancer markers of the 1990s. Clin Lab Med 1990; 10:1-37. 5. Hirai H et. al., Some chemical, experimental and clinical investigations on alpha \nfetoprotein. In: Hirai H, Miyaji T, eds. Alpha-fetoprotein and hepatoma. Gann Monogr 1973; 14:19-34.