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a-Keto Acid Dehydrogenase Complex, Branched-Chain (BCKDH)

Cat no: K0290

a-Keto Acid Dehydrogenase Complex, Branched-Chain (BCKDH)

Mitochondial enzymes regulated by reversible phosphorylation. Catalyzes the oxidative decarboxylation of the branched-chain a-keto acids derived from the essential amino acids, leucine, isoleucine and valine. Purified preparations undergo phosphorylation because endogenous kinase is tightly associated with the complex.\n\nSpecific Activity: \n~8-10 mmol of NADH formed per min per mg of protein with a-ketoisovalerate as substrate. \n\nMolecular Weight:\n3200kD by sedimentation equilibrium centrifugation. Subunit apparent MW: 52kD (E2), 46kD (E1a), 35kD (E1b)\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and store at -70 degrees C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

K0290

Size

10ug

Form

Supplied as a liquid in 50mM Tris HCl, pH 7.0, 14mM b-mercaptoethanol, 1mM benzamidine, 0.1mM PMSF, 1mM EDTA, 10% glycerol.

Purity

~90% (SDS-PAGE)

References

1. Methods Enzymol 166: 321

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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