a-Methylacyl CoA Racemase, Recombinant, Human (Alpha Methylacyl CoA Racemase, Alpha Methylacyl Coenzyme A Racemase, AMACR, 2-Arylpropionyl CoA Epimerase, 2-Methylacyl CoA Racemase, EC 5.1.99.4, Macr1, Methylacyl CoA Racemase alpha, P504S, RACE)

504s / AMACR a-methyl-CoA Racemase is an essential enzyme in the b-oxidation of branched-chain fatty acids, it is responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers, which are the only stereoisomers that can be degraded via peroxisomal beta-oxidation. High expression of P504s protein is found in prostatic adenocarcinoma but not in benign prostate tissue by immunohistochemical staining in paraffin-ebmbedded tissue. The expression of P504s is also detected in two premalignant lesions of the prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Inhibition Assays. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M3390

Size

2ug

Form

Supplied as a liquid in 50mM Tris-Acetate, pH 7.5, 1mM EDTA, 20% glycerol.

Purity

~90% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Alpha -methylacyl-CoA racemase from mycobacterium tuberculosis-mutational and structural characterization of the active site and the fold. Savolainen K, Bhaumik P, Kotti TJ, J Biol Chem 2005 Jan 4; 2. Alpha-methylacyl-CoA racemase (AMACR) expression in normal prostatic glands and high-grade prostatic intraepithelial neoplasia (HGPIN): Association with diagnosis of prostate cancer. Ananthanarayanan V, Deaton RJ, Pins MR, Prostate 2004 Dec 15; 3. Diagnostic utility of alpha-methylacyl CoA racemase (P504S) on prostate needle biopsy. Jiang Z, Woda BA, Adv Anat Pathol 2004 Nov;11(6):316-21 4. [alpha-methylacyl-CoA racemase: a useful marker for diagnosis of prostatic carcinoma.] Jiang Z, Ma K, Yang XJ, Zhonghua Bing Li Xue Za Zhi 2004 Oct;33(5):401-3 5. Utility of immunohistochemistry for alpha-methylacyl-CoA racemase in distinguishing atrophic prostate cancer from benign atrophy. Farinola MA, Epstein JI, Hum Pathol 2004 Oct;35(10):1272-8 6. [Detection of P504S (alpha-methylacyl-CoA racemase) in prostatic adenocarcinomas] Xiao Y, Chen J, Cao JL, Zhonghua Yi Xue Za Zhi 2004 Aug 17;84(16):1362-6.