Alpha1-Antichymotrypsin (AACT) is a serine proteinase inhibitor and minor acute phase protein with a molecular weight of ~68kD, occurring in serum at 250-500mg/L. AACT appears to inhibit chymotrypsin specifically.
Applications:
Suitable for use in ELISA, Immunoelectrophoresis and Single and Double Radial Immunodiffusion. Other applications not tested.
Recommended Dilution:
Immunoelectrophoresis: 2ul plasma or equivalent against 120ul antiserum
Double Radial Immunodiffusion: Use a rosette arrangement with 10ul antiserum in 3mm diameter center well and 2ul plasma samples (neat and serially diluted) in 2mm diameter
peripheral wells.
Single Radial Immunodiffusion: 1% in the gel.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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