a1-Antitrypsin (AAT, alpha-1-AT, alpha1 Proteinase Inhibitor, alpha1 PI) (HRP)

Alpha-1-antitrypsin (AAT) consists of a single polypeptide chain and has a molecular weight of 51kD. It exists in a number of genetic variants. MM variant is the most common. Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leukocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of AAT, these enzymes are not inhibited and they may digest pulmonary parenchyma. AAT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in AAT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis. In colorectal and lung carcinomas, AAT has also related to their invasive and metastatic capacity. The average level of AAT in plasma is 1.3g/L. \n\nApplications:\nSuitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:1000-1:5000\nELISA: 1:4000-1:20,000\nImmunohistochemistry: 1:500-1:2500\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

A2298-28

Size

1mg

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% gentamycin sulfate. Labeled with horseradish peroxidase (HRP).

P Type

Pab

Purity

Purified by delipidation, salt fractionation, ion exchange chromatography and dialysis.

Isotype

IgG

References

US Biological application references: 1. Huang, T.-K. et al., (2008) Biotechnology and Bioengineering 102:508-520. 2. Huang, T.-K. et al., (2010) Biotechnology and Bioengineering 106; 408-421. General Reference(s): 1. Farr & Nakane, J. Immunol. Methods 47: 129-144 (1981).

Additional Info

Recognizes human alpha1-Antitrypsin. Assay by IEP resulted in a single precipitin arc against anti-peroxidase and anti-goat serum as well as purified and partially purified alpha1-Antitrypsin (human plasma).