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a1-Antitrypsin (AAT, alpha-1-AT) (HRP)

Cat no: A2298-29H

a1-Antitrypsin (AAT, alpha-1-AT) (HRP)

Alpha-1-antitrypsin (AAT) consisting of a single polypeptide chain and has a molecular weight of 51kD. It exists in a number of genetic variants. MM variant is the most common. Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leukocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of AAT, these enzymes are not inhibited and they may digest pulmonary parenchyma. AAT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in AAT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis. In colorectal and lung carcinomas, AAT has also related to their invasive and metastatic capacity. The average level of AAT in plasma is 1.3g/L. \n\nApplications: Suitable for use in ELISA, Immunohistochemistry.\n\nRecommended Dilution:\nImmunohistochemistry: (frozen)- 1:20-1:40\nELISA: 1:6400-1:12800\nOptimal working dilutions to be determined by researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2298-29H

Size

1ml

Applications

ELISA, IHC

Hosts

Sheep

Reactivities

Hum

Form

Supplied as a liquid in PBS with 0.05% Proclin TM 300, 0.002% Methylisothiazolone, 0.002% Bromonitrodioxane.

P Type

Pab

Purity

Purified IgG was prepared from whole serum by ion exchange chromatography.

Isotype

IgG

Additional Info

Recognizes alpha 1-antitrypsin, a 51kD glycoprotein which demonstrates serine protease inhibitor activity.

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