a1-Antitrypsin (AAT, alpha1 Proteinase Inhibitor, alpha1 PI)

a1-antitrypsin (a-1 AT) is a single polypeptide chain, a typical member of the serine family (serine protease inhibitors), inhibiting the enzyme trypsin. a-1 AT is a glycoprotein synthesized in the liver, and is genetically polymorphic due to the presence of over 20 alleles; individuals appropriately homozygous are deficient in a-1 trypsin and are predisposed to pulmonary emphysema and juvenile hepatic cirrhosis because of alterations in the amino acid and sialic acid components of the glycoprotein (1).The molecular mass of a-1 AT is 51kD.\n\nApplications: \nSuitable for use in ELISA, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:8000\nWestern Blot: 1:8000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A2298-38A

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 15mM sodium azide.\n

P Type

Mab

Purity

Purified by Protein A/G affinity chromatography from culture supernatant.

Isotype

IgG2a,k

References

1. Stedman's Medical Dictionary (1990) Williams & Wilkins, Baltimore, USA.

Additional Info

Has specificity for native human a-1 AT. Epitope specificity differs from that of clone 6F9.\nReacts strongly with native a-1 AT. Strong reaction is seen in ELISA with native a-1 AT directly coated onto the microtiter well. In Western blotting after SDS-PAGE, this antibody reacts with native a-1 AT in reduced as well as non-reduced form.