a1-Antitrypsin (AAT, alpha1 Proteinase Inhibitor, alpha1 PI) (Glycerol, BSA and Azide Free)

a1Antitrypsin (a1AT), also known as a1Proteinase inhibitor \n(a1PI), is the most abundant protease inhibitor in blood and a member of the SERPIN family of proteinase inhibitors. Serum levels are typically 1.3mg/ml (2.5uM). a1AT is an acute phase protein and concentrations can rise four-fold during inflammatory episodes or tissue injury. Low levels in circulation have been associated with pulmonary disease such as emphysema. a1AT is a single chain molecule (52kD) that is produced primarily in the liver and to a lesser extent by blood monocytes and intestinal epithelium. Based on association rates, the primary target enzyme for a1AT is believed to be neutrophil elastase. a1AT is a broad-spectrum inhibitor for many serine proteinases. The main role of a1AT in vivo is likely that of a

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SPECIFICATIONS

Catalog Number

A2298-27B1

Size

500ug

Applications

ELISA

Hosts

Sheep

Reactivities

Hum

Form

Supplied in TBS, pH 7.5. Glycerol free. No preservative added. No stabilizing proteins added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Johnson, D., Travis, J.; Oxidative Inactivation of Human alpha-1-Proteinase Inhibitor; JBC 254: 4022-4026 (1979). 2. Travis, J., Johnson, D.; Human alpha-1-Proteinase Inhibitor; Methods in Enzymology 80: 754-765 (1981). 3. Heeb, M.J., Griffin, J.H.; Physiologic Inhibition of Human Activated Protein C by alpha-1-Trypsin Inhibitor; JBC 263: 1163-11616 (1988). 4. Scott, C.F., Schapira, M., James, H.L,, Cohen, A.B., Colman, R.W.; The Inactivation of Factor XIa by plasma protease inhibitors: Predominant role of alpha-1protease inhibitor and protective effect of high molecular weight kininogen. J. Clin. Invest. 69: 844 (1982).

Additional Info

Specific for alpha1AT as demonstrated by immunoelectrophoresis. A single precipitin arc is observed with normal plasma, of identical mobility to that obtained with purified alpha1AT.