a1-Antitrypsin (AAT, alpha1 Proteinase Inhibitor, alpha1 PI) (HRP)

a1-Antitrypsin (a1-AT) is a single polypeptide chain, a typical member of the serine family (serine protease inhibitors), inhibiting the enzyme trypsin. a1-AT is a glycoprotein synthesized in the liver, and is genetically polymorphic due to the presence of over 20 alleles. Individuals appropriately homozygous are deficient in a1-trypsin and are predisposed to pulmonary emphysema and juvenile hepatic cirrhosis because of alterations in the amino acid and sialic acid components of the glycoprotein. The molecular mass of a1-AT is ~52kD.\n\nApplications:\nSuitable for use in ELISA and Immunoelectrophoresis. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nRz Ratio:\nReinheitszahl; A403/A280 = 0.456 \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

A2298-28A

Size

100ug

Applications

ELISA

Hosts

Sheep

Reactivities

Hum

Form

Supplied in buffered stabilizer, 50% glycerol. No preservatives added. Labeled with HRP.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Johnson, D. & Travis, J., J. Biol. Chem. 254: 4022-4026 (1979). 2. Travis, J. & Johnson, D., Methods in Enzymology 80: 754-765 (1981). 3. Heeb, M.J. & Griffin, J.H., J. Biol. Chem. 263: 11,613-11,616 (1988). 4. Scott, C.F., et al., J. Clin. Invest. 69: 844 (1982).

Additional Info

Recognizes human alpha1-Antitrypsin.