a2-Macroglobulin

Alpha-2-Macroglobulin detects Macroglobulin. Alpha-2-Macroglobulin is a large plasma protein found in the blood. It is produced by the liver, and is a major component of the alpha-2 band in protein electrophoresis. Alpha 2-Macroglobulin is the largest major nonimmunoglobulin protein in plasma. The alpha 2-macroglobulin molecule is synthesized mainly in liver, but also locally by macrophages, fibroblasts, and adrenocortical cells. Alpha 2 macroglobulin acts as an antiprotease and is able to inactivate an enormous variety of proteinases. It functions as an inhibitor of fibrinolysis by inhibiting plasmin and kallikrein. It functions as an inhibitor of coagulation by inhibiting thrombin. Alpha 2-macroglobulin may act as a carrier protein because it also binds to numerous growth factors and cytokines, such as platelet-derived growth factor, basic fibroblast growth factor, TGF-b, insulin, and IL-1b. No specific deficiency with associated disease has been recognized, and no disease state is attributed to low concentrations of Alpha 2 macroglobulin. The concentration of alpha 2 macroglobulin rises 10-fold or more in the nephrotic syndrome when other lower molecular weight proteins are lost in the urine. The loss of alpha 2 macroglobulin into urine is prevented by its large size. The net result is that alpha 2 macroglobulin reaches serum levels equal to or greater than those of albumin in the nephrotic syndrome, which has the effect of maintaining oncotic pressure. Antialpha-2-macroglobulin is ideal for investigators involved in serum component protein research.\n\nApplications: \nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions: \nWestern Blot: 1:1000\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

M1150-04

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a lyophillized powder from PBSl, pH 7.2, 0.01% sodium azide. Reconstitute with 100ul sterile ddH2O.

P Type

Pab

Purity

Puri?ed by delipidation, fractionation and Ion Exchange chromatography.

Isotype

IgG

References

1. Armstrong PB, Quigley JP (1999). \"Alpha2-macroglobulin: an evolutionarily conserved arm of the innate immune system\". Dev. Comp. Immunol. 23 (4-5): 375

Additional Info

Recognizes human a2-Macroglobulin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as puri?ed and partially puri?ed a2-Macroglobulin human plasma.