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A20 Zinc Finger Protein

Cat no: A0000-10

A20 Zinc Finger Protein

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The zinc finger protein A20 is a tumor necrosis factor and interleukin 1 (IL-1)-inducible protein that negatively regulates NF-kB gene expression. A20 inhibits NF-kB dependent gene expression by interfering with a novel TNF-induced and RIP- or TRAF2-mediated pathway that is different from the NIK-IkB kinase pathway. A20 is expressed at low levels in most organs, the highest expression levels being in lymphoid tissues. \n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunoprecipitation and Immunofluorescence. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: Intracellular\nWestern Blot: 2-4ug/ml\nImmunoprecipitation: 1-2ug/ml\nImmunohistochemistry: Paraffin \nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nJurkat cells, THP1\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0000-10

Size

100ug

Applications

FC, IF, IHC, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.05% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1,k

References

US Biological Application References: 1. Wang, J. et al., J. Immunology 183: 1384

Additional Info

Recognizes human A20 Zinc Finger Protein. \nSpecies Crossreactivity: mouse and rat.

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