A2M/a2-Macroglobulin BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human A2M/a2-Macroglobulin in cell culture supernates, serum and plasma (heparin, EDTA).\n\nAlpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.\n\nAssay Range:\n625pg/ml-40,000pg/ml\n\nSensitivity:\n< 20 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP01023\n\nEntrez Gene ID:\n2\n\nKit Components:\n96-well plate pre-coated with anti-A2M/a2-Macroglobulin, Human\nLyophilized recombinant A2M/a2-Macroglobulin, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143702

Size

96Tests

References

1. Bian, L., Yang, J. D., Guo, T. W., Duan, Y., Qin, W., Sun, Y., Feng, G. Y., He, L. Association study of the A2M and LRP1 genes with Alzheimer disease in the Han Chinese. Biol. Psychiat. 58: 731-737, 2005.\n2. Flachsbart, F., Caliebe, A., Nothnagel, M., Kleindorp, R., Nikolaus, S., Schreiber, S., Nebel, A. Depletion of potential A2M risk haplotype for Alzheimer's disease in long-lived individuals. Europ. J. Hum. Genet. 18: 59-61, 2010. \n3. Zappia, M., Manna, I., Serra, P., Cittadella, R., Andreoli, V., La Russa, A., Annesi, F., Spadafora, P., Romeo, N., Nicoletti, G., Messina, D., Gambardella, A., Quattrone, A. Increased risk for Alzheimer disease with the interaction of MPO and A2M polymorphisms. Arch. Neurol. 61: 341-344, 2004.\n