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A33 (GPA33, Glycoprotein A33 (transmembrane) Cell surface A33 antigen precursor, Glycoprotein A33, MGC129986, MGC129987)

Cat no: A0000-33B

A33 (GPA33, Glycoprotein A33 (transmembrane) Cell surface A33 antigen precursor, Glycoprotein A33, MGC129986, MGC129987)

Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214aa in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells. \n\nApplications: \nSuitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nFlow Cytometry: 2.5ug stains 10e6 cells from the HT-29 human colon carcinoma cell line.\nWestern Blot: 1ug/ml\nImmunohistochemistry: 8-25ug/ml stains immersion fixed paraffin-embedded sectionf of human colon and human colon cancer tissue subjected to antigen retrieval, basic.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0000-33B

Size

100ug

Applications

ELISA, FC, IHC, WB

Hosts

Rat

Reactivities

Hum

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2a

Additional Info

Recognizes human A33. Does not crossreact with recombinant mouse A33.

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