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A33 (GPA33, Glycoprotein A33 Transmembrane Cell surface A33 antigen precursor, MGC129986, MGC129987) (Biotin)

Cat no: A0000-33C

A33 (GPA33, Glycoprotein A33 Transmembrane Cell surface A33 antigen precursor, MGC129986, MGC129987) (Biotin)

Human A33, also known as GPA33, is a 43kD type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells. \n\nApplications: \nSuitable for use in Western Blot and Flow Cytometry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2ug/ml\nFlow Cytometry: 50ug/ml. Use 10ul of diluted solution/1-2.510e5 cells in <200ul total volume. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile TBS, pH 7.3, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0000-33C

Size

50ug

Applications

FC, WB

Hosts

Goat

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS with BSA. Labeled with Biotin. Reconstitute with 1ml TBS, pH 7.3, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.\n\n

Isotype

IgG

Additional Info

Recognizes human A33. Species crossreactivity: ~10% crossreactivity with recombinant mouse A33.

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