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A431 Calyculin A Control Lysate

Cat no: A0001-52

A431 Calyculin A Control Lysate

Calyculin A is a serine/threonine phosphatase inhibitor that inhibits the activity of protein phosphatases PP1 and PP2A. Human carcinoma A431 cells treated with calyculin A for 30 minutes can undergo significant threonine phosphorylation, as shown by western blotting using anti-Phospho-Akt (Thr-34), as compared to untreated, control cell lysates.\nConfluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated or treated with Calyculin A at a final concentration of 10nM for 30 minutes at 37 degrees C. Cells were lysed in 1% SDS, 1.0mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.\n\nApplications:\nSDS-PAGE/Western blotting 20ml/well\nEnd user should determine optimal quantity for their particular applications and experiments.

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SPECIFICATIONS

Catalog Number

A0001-52

Size

100ug

Applications

WB

Form

Supplied in electrophoresis sample buffer (62.5mM Tris pH 7.2, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% b-mercaptoethanol).

References

1999 Resjo, S. et al. Biochem. J. 341:839-845.\n1989 Ishihara, H. et al. Biochem. Biosphys. Res. Commun. 159(3):871-877

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