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AADAC (Arylacetamide Deacetylase, DAC)

Cat no: 122776

AADAC (Arylacetamide Deacetylase, DAC)

Arylacetamide deacetylation is an important enzyme activity in the metabolic activation of arylamine substrates to ultimate carcinogens. Displays major serine hydrolase activity in liver microsomes. Hydrolyzes also flutamide, which is an antiandrogen drug used for the treatment of prostate cancer that occasionally causes severe hepatotoxicity. Displays cellular triglyceride lipase activity in liver. Increases intracellular fatty acids derived from hydrolysis of newly formed triglyceride stores.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nAA Sequence:\nQLLDDPDVKIKLKIQSLIYPALQPLDVDLPSYQENSNFLFLSKSLMVRFWSEYFTTDRSLEKAMLSRQHVPVESSHLFKFINWSSLLPERFIKGHVYNNP\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

122776

Size

100ug

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG2b,k

References

1. Shimizu M, Fukami T, Kobayashi Y, Takamiya M, Aoki Y, Nakajima M, Yokoi T.Drug Metab Dispos. 2012 Mar 13. [Epub ahead of print]. 2. Kobayashi Y, Fukami T, Nakajima A, Watanabe A, Nakajima M, Yokoi T.Drug Metab Dispos. 2012 Apr;40(4):671-9. Epub 2011 Dec 29.. 3. Watanabe A, Fukami T, Nakajima M, Takamiya M, Aoki Y, Yokoi T.Drug Metab Dispos. 2009 Jul;37(7):1513-20. Epub 2009 Apr 1.

Additional Info

Recognizes human AADAC.

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