During the biopharmaceutical manufacturing process, the residue of Host Cell Proteins (HCPs) is an unavoidable quality control issue. These residual proteins may affect the safety, stability, and biological activity of the drug. Therefore, precise and comprehensive analysis of HCPs is crucial. Affinity Antibody Extraction-Two Dimensional electrophoresis (AAE-2D) is a technique primarily used for the efficient detection and analysis of proteins.