AatII

Sequence:\n5'-G A C G T^C-3' \n3'-C^T G C A G-5'\n\nSupplied With:\nRestriction Enzyme Buffer A (R1625): (for 100% AatII digestion) 33mM Tris-acetate (pH 7.9), 10mM magnesium acetate, 66mM potassium acetate and 0.1mg/ml BSA. Incubate at 37 degrees C.\n\nDiluent Buffer: \n10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM potassium chloride, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.\n\nStorage Buffer: \n10mM Tris-HCl (pH 7.4 at 25 degrees C), 100mM potassium chloride, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.\n\nOverdigestion Assay: \nNo detectable change in the specific fragmentation pattern is observed after a 160-fold overdigestion with AatII \n(10u/ug lambda DNA x 16 hours).\n\nLigation/Recutting Assay: \nAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with AatII more than 95% of the digested DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these sites can be recut. \n\nLabeled Oligonucleotide (LO) Assay: \nNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.\n\nStorage and Stability:\n\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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