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ABC B5 (ATP Binding Cassette Sub-family B (MDR/TAP) Member 5, ATP-binding Cassette Sub-family B Member 5, ABCB 5, ABCB5, ABCB5alpha, ABCB5beta, EST422562, P-glycoprotein ABCB5)

Cat no: A0004-02Y1

ABC B5 (ATP Binding Cassette Sub-family B (MDR/TAP) Member 5, ATP-binding Cassette Sub-family B Member 5, ABCB 5, ABCB5, ABCB5alpha, ABCB5beta, EST422562, P-glycoprotein ABCB5)

ABCB5 (ATP-binding cassette, subfamily B [DR/TAP] member 5) is a 90kD (predicted MW) member of the human P-glycoprotein family of molecules. It is expressed by CD133+ pluripotent pigment stem cells, where it serves as a marker for cells that show a high incidence of polyploidy. Human ABCB5 is 812aa in length. It is possibly a five transmembrane protein with a 247aa N-terminal extracellular domain (ECD) aa1-247 and a 283aa C-terminal cytoplasmic region aa530-812. The ECD contains one ABC transporter aa2-177, while the cytoplasmic region contains a second ABC transporter aa570-808. There are two potential splice forms. One shows a seven aa substitution for aa125-812, while another possesses an alternative start site 445aa upstream of the standard site. Over aa141-247, human ABCB5 shares 64aa identity with mouse ABCB5.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.03-0.1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nlysates of A431\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0004-02Y1

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human ABCB5.

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