ABCC4 (ATP-binding cassette sub-family C member 4, MRP/cMOAT-related ABC transporter, Multi-specific organic anion transporter B)

ABCC4(Atp-binding cassette, subfamily c, member 4) also known as MRP4 or MOATB, is a protein that in humans is encoded by the ABCC4 gene.The ABCC4 belongs to a large family of transmembrane proteins involved in active transport of substrates out of cells. By radiation hybrid analysis, Kool et al. (1997) mapped the ABCC4 gene to chromosome 13. Using FISH, Lee et al. (1998) refined the localization to chromosome 13q32. ABCC4 acts as an independent regulator of intracellular cyclic nucleotide levels and as a mediator of cAMP-dependent signal transduction to the nucleus. The antiproliferative effect of MRP4 inhibition was related to cAMP-dependent PKA activation and CREB phosphorylation. Pharmacologic inhibition of MRP4 activity or knockdown of MRP4 via RNA interference resulted in reduced migration of DCs from human skin explants and of in vitro-generated Langerhans cells. Van de Ven et al. (2008) concluded that MRP4 contributes to migration of DCs toward draining lymph nodes and therefore has a role in the initiation of an immune response.\n\nUniProt Number:\nO15439\n\nGene ID:\nABCC4\n\nApplications:\nSuitable for use in Western Blot.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

144105

Size

100ug

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Kool, M., de Haas, M., Scheffer, G. L., Scheper, R. J., van Eijk, M. J., Juijn, J. A., Baas, F., Borst, P. Analysis of expression of cMOAT (MRP2), MRP3, MRP4, and MRP5, homologues of the multidrug resistance-associated protein gene (MRP1), in human cancer cell lines. Cancer Res. 57: 3537-3547, 1997.\n2. Lee, K., Belinsky, M. G., Bell, D. W., Testa, J. R., Kruh, G. D. Isolation of MOAT-B, a widely expressed multidrug resistance-associated protein/canalicular multispecific organic anion transporter-related transporter. Cancer Res. 58: 2741-2747, 1998.\n3. van de Ven, R., Scheffer, G. L., Reurs, A. W., Lindenberg, J. J., Oerlemans, R., Jansen, G., Gillet, J.-P., Glasgow, J. N., Pereboev, A., Curiel, D. T., Scheper, R. J., de Gruijl, T. D. A role for multidrug resistance protein 4 (MRP4;ABCC4) in human dendritic cell migration.Blood 112: 2353-2359, 2008.\n

Additional Info

Recognizes human, mouse and rat ABCC4. No crossreactivity with other proteins.