ABCG1 (ATP-Binding Cassette (ABC) Sub-family G Member 1, AbC8, ATP-Binding Cassette Tansporter 8, White Protein Homolog, WHT1)

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. The ABC transporter ABCG1 (ABC8) is expressed on the cell surface and in intracellular compartments of cholesterol-laden macrophages and is induced in monocyte-derived macrophages during cholesterol influx mediated by acetylated low-density lipoprotein. In contrast, lipid efflux in cholesterol-laden macrophages, mediated by the cholesterol acceptor HDL(3), suppresses the expression of ABCG1.\n\nApplications: \nSuitable for use in Western Blo and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: Theoretical molecular weight of ABCG1 is ~75.6kD,\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0004-12B

Size

100ul

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

As reported

P Type

Pab

Purity

Purified by peptide immunoaffinity chromatography.

References

1. Avallone, R., Demers, A., Rodrigue-Way, A., Bujold, K., Harb, D., Anghel, S., Wahli, W., Marleau, S., Ong, H., and Tremblay, A. (2006) A Growth Hormone-Releasing Peptide that Binds Scavenger Receptor CD36 and Ghrelin Receptor Up-Regulates Sterol Transporters and Cholesterol Efflux in Macrophages through a Peroxisome Proliferator-Activated Receptor {gamma}-Dependent Pathway, 20, 3165-3178. 2. Li, T., Chen, W., and Chiang, J. Y. L. (2007) PXR induces CYP27A1 and regulates cholesterol metabolism in the intestine, 48, 373-384. 3. Ranalletta, M., Wang, N., Han, S., Yvan-Charvet, L., Welch, C., and Tall, A. R. (2006) Decreased Atherosclerosis in Low-Density Lipoprotein Receptor Knockout Mice Transplanted With Abcg1-/- Bone Marrow, 26, 2308-2315. 4. Tansley, G. H., Burgess, B. L., Bryan, M. T., Su, Y., Hirsch-Reinshagen, V., Pearce, J., Chan, J. Y., Wilkinson, A., Evans, J., Naus, K. E., McIsaac, S., Bromley, K., Song, W., Yang, H.-C., Wang, N., DeMattos, R. B., and Wellington, C. L. (2007) The cholesterol transporter ABCG1 modulates the subcellular distribution and proteolytic processing of beta -amyloid precursor protein, M600542-JLR600200. 5. Wang, M.-D., Kiss, R. S., Franklin, V., McBride, H. M., Whitman, S. C., and Marcel, Y. L. (2007) Different cellular traffic of LDL-cholesterol and acetylated LDL-cholesterol leads to distinct reverse cholesterol transport pathways, 48, 633-645.

Additional Info

The specificity of this antibody is for ABCG1. Species Crossreactivity: This antibody reacts with mouse and human protein. Other species have not been tested. There is 100%/100% identity/homology of the immunogen used for this production and the human protein sequence. There is 84%/89% for mouse and 78%/88% for rat.

Alternative Names

ABC8, ABCG1, MGC34313, WHITE, WHITE1, WHT1