ABCG3 (ATP-binding Cassette (ABC) Transporter 3)

The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified.\nABCG3 protein is a 650aa protein in mouse, highly expressed in the thymus and spleen. It seems to have defective ATP binding region, which suggests the ABCG3 protein may not bind or hydrolyze ATP. Therefore, it has to dimerize with another subunit to form a functional transporter. It is unique among ABC genes in not having several highly conserved residues in the A and C domains of NBF. ABCG3 is the most closely related to ABCG2 with 54% homology overall, 64% in the NBF and 50% in the TM region.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested. \n\nRecommended Dilutions: \nWestern Blot: 1-10ug/ml (ECL)\nELISA: 0.5-1ug/ml; Use control peptide (Cat #A0004-04U1) to coat ELISA plates at 1ug/ml.\nOptimal dilutions to be deermined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

A0004-04U

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ozvegy, C., et al., JBC 277(50): 47,980-47,980 (2002). 2. Mickley, L., et al., Mammalian Genome 12: 86-88 (2001). 3. Doyle, L.A., PNAS 95: 15,665-15,670 (1998).

Additional Info

Recognizes mouse ABCG3.