ABCG8 (ATP binding cassette, subfamily G, member 8)

ATP-binding cassette (ABC) transporter genes are involved in the regulation of the amount of dietary cholesterol retained in the body. ABCG8, expressed at high levels in the liver and intestine, normally cooperates with ABCG5 to limit intestinal absorption and promote biliary excretion of sterols. The mutated form of this transporter can lead to sterol accumulation and atherosclerosis or sitosterolemia, a rare autosomal recessive disorder, characterized by hyperabsorption of sterols and the inability to excrete sterols into bile. \n\nApplication(s): \nA doublet is seen at ~67 and ~72kD in Western analysis. Longer exposure times are needed to visualize the wild type ABCG8. \n\nRecommended Dilution: \nWestern Blot -1:250 (ECL) \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0004-10

Size

200ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid with sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

General: 1. Patel, S.B., et al. Two genes that map to the STSL locus cause sitosterolemia: genomic structure and spectrum of mutations involving sterolin-1 and sterolin-2, encoded by ABCG5 and ABCG8,respectively. Am. J. Hum. Genet., 69: 278-290, 2001. 2. Garcia, C.K., et al. Autosomal recessive hypercholesterolemia caused by mutations in a putative LDL receptor adaptor protein. Science, 292: 1394-1398, 2001. 3. Schmitz, G., et al. Role of ABCG1 and other ABCG family members in lipid metabolism. J. Lipid Res. 42: 1513-1519, 2001.

Additional Info

Specific for human ABCG8. Species Crossreactivity: It is likely to work in mouse due to sequence homology.