ABCG8 (ATP binding cassette, subfamily G, member 8)

The ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes, host-defense mechanism to xenobiotics. The first known members were P-glycoprotein (P-gp) and multidrug resistant protein (MRP), cause multidrug resistance when transfected into drug-sensitive cells. In addition, increasing numbers of ABC proteins have recently been identified. The human ABCG1 (ABC, subfamily G, member 1) gene encodes a member of ABC superfamily that mediates the ATP-dependent translocation of variety of amphiphilic and lipophilic molecules. ABCG proteins were able to transport substances across cellular membranes and against concentration gradient they require an input of energy, which requires the hydrolysis of ATP, directly or indirectly. Rat ABCG8 protein is alternatively spliced: A full length isoforms 3 (694-aa), isoforms 2 (671-aa; missing 56-77 aa, and 398-aa); isoforms 1 (672aa, missing 56-77 aa). Actual expression of various protein isoforms and their physiological relevance is not understood. ABCG8 may form heterodimers with ABCG5 or be tightly coupled to ABCG5 along a pathway regulating dietary-sterol absorption and excretion. ABCG8 protein is highly expressed in liver, intestine and colon. Defects in ABCG8 cause sitosterolemia.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1-10ug/ml (ECL)\nELISA: 0.5-1ug/ml. Use Control Peptide (Cat #A0004-11A) tocoat plate at 1ug/ml.\nOptimal dilution determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0004-11

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

Lu. K et al (2001) Am. J. Hum. Genet. 69 (2), 278-290; Berge, K. E et al (2000) Science 290 (5497), 1771-1775; Lee, M. H et al (2001) Nat. Genet. 27 (1), 79-83; L. Austin Doyle (1998) PNAS Vol. 95, 15665-15670.

Additional Info

Recognizes rat ABCG8. \nSpecies sequence homology: rmouse, 65%. No significant homology in human ABCG8.