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ABI1 (Abl Interactor 1, Abelson Interactor 1, Abi-1, Spectrin SH3 Domain-binding Protein 1, Eps8 SH3 Domain-binding Protein, Eps8-binding Protein, e3B1, Nap1-binding Protein, Nap1BP, Abl-binding Protein 4, AblBP4, SSH3BP1)

Cat no: A0007-34G

ABI1 (Abl Interactor 1, Abelson Interactor 1, Abi-1, Spectrin SH3 Domain-binding Protein 1, Eps8 SH3 Domain-binding Protein, Eps8-binding Protein, e3B1, Nap1-binding Protein, Nap1BP, Abl-binding Protein 4, AblBP4, SSH3BP1)

ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1 participate in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities. ABI1 dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins. ABI1 plays a role in the leukemogenesis by translocating to MLL. \n\nApplications:\nSuitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-2ug/ml \nImmunohistochemistry (frozen/paraffin): 0.5-1ug/ml \nImmunocytochemistry: 0.5-1ug/ml \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile dH20. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A0007-34G

Size

100ug

Applications

ICC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes human ABI1. Species Cross-Reactivity: mouse, rat.

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