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ABIN-1 (A-20 Binding Inhibitor of Nuclear Factor-kappaB (NF-kB) Activation, HIV-1 Nef-interacting Protein, KIAA0113, Nef-associated Factor 1, Naf1, Nip40-1, TNFAIP3 Interacting Protein 1, TNIP1, Virion-associated Nuclear Shuttling Protein, VAN, hVAN)

Cat no: A0004-74

ABIN-1 (A-20 Binding Inhibitor of Nuclear Factor-kappaB (NF-kB) Activation, HIV-1 Nef-interacting Protein, KIAA0113, Nef-associated Factor 1, Naf1, Nip40-1, TNFAIP3 Interacting Protein 1, TNIP1, Virion-associated Nuclear Shuttling Protein, VAN, hVAN)

The ABIN family (ABIN-1, -2, and -3) is a group of adaptor proteins that associate and cooperate with A20/TNFAIP3 (1), a ubiquitin editing protein that inhibits the key inflammatory transcription factor NF-kB (2-4). Mechanistically, A20 acts by regulating the ubiquitination of the kinase RIP, which leads to inhibition of the IKK complex (5).\nABIN-1 (Naf1/TNIP1) was identified based on its binding to A20 (6), as well as the HIV protein Nef-1 (7). Overexpression of ABIN-1 inhibits NF-kB activation by a number of stimuli including TNF, IL-1, and LPS. It is widely expressed and can be induced by NF-kB, providing a negative feedback loop of NF-kB signaling. In addition to binding to A20, ABIN-1 can function on other key components of NF-kB signaling including IKKg/NEMO (8) and NF-kB family members p100 and p105 (9), and can also inhibit ERK signaling (10). Knockout mice of ABIN-1 are embryonic lethal, with fetal liver apoptosis, anemia and hypoplasia (11). ABIN-1 deficient cells are hypersensitive to TNF-mediated apoptosis.\n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nmmunoprecipitation: 1:100\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0004-74

Size

100ul

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

Additional Info

Recognizes endogenous levels of human total ABIN-1 protein. Species Crossreactivity: mouse, rat. Species sequence homology: monkey.

Alternative Names

TNFAIP3-interacting Protein 1

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