ABIN3 (TNFAIP3-interacting Protein 3, A20-binding Inhibitor of NF-kappa-B Activation 3, ABIN-3, Listeria-induced Gene Protein, TNIP3, LIND)

The nuclear factor NF-kB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-kB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-kB activation. ABIN3 is a 39kD protein that negatively regulates NF-kB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-kB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-kB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.\n\nApplications:\nSuitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nImmunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Spleen Tissue Lysate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

146495

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Verstrepen L, Carpentier I, Verhelst K, et al. Abins: A20 binding inhibitors of NF?B and apoptosis signaling. Biochem. Pharmacol. 2009; 78:105-14. 2. Bouwmeester T, Bauch A, Ruffner H, et al. A physical and functional map of the human TNF?/NF?B signal transduction pathway. Nat. Cell Biol. 2004; 6:97-105.\n3. Wullaert A, Verstrepen L, Van Huffel S, et al. LIND/Abin-3 is a novel lipopolysaccharide-inducible inhibitor of NF?B activation. J. Biol. Chem. 2007; 282:81-90. 4. Weaver BK, Bohn E, Judd BA, et al. Abin-3: a molecular basis for species divergence in interleukin-10-induced anti-inflammatory actions. Mol. Cell. Biol. 2007; 27:4603-16.

Additional Info

Recognizes human ABIN3. Species Crossreactivity: mouse.