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Abl, Human, Active

Cat no: A0005-10

Abl, Human, Active

N-terminal His6-tagged recombinant human Abl, residues 27-end, expressed by baculovirus in Sf21 insect cells.\n\nSpecific Activity: ~500U/mg\n\nUnit Definition: \nOne unit of Abl activity is defined as 1nmol phosphate incorporated into 50uM Abltide (EAIYAAPFAKKK) per minute at 30 degrees C with a final ATP concentration of 100uM.\n\nQuality Control Testing:\nMS Tryptic Fingerprint: Confirmed identity as Abl with 20% amino acid coverage of the translated sequence\n\nStorage and Stability:\nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

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SPECIFICATIONS

Catalog Number

A0005-10

Size

5ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol.

Purity

~40% by SDS-PAGE and Coomassie blue staining. Purified using Ni/NTA-agarose purification.

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Applications

ELISA

Hosts

Goat

Reactivities

Hum, Mouse, Rat

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