ACAT1 (Acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, ACAT, acetyl-Coenzyme A acetyltransferase 1, ACAT1)

This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone. \n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications have not been tested. \n\nRecommended Dilutions: \nPeptide ELISA Titer: 1:2000\nWestern Blot: 0.1-0.3ug/ml ~45kD band observed in Human, Mouse and Rat Liver lysates (calculated MW of 45.2kD according to Human NP_000010.1 and 44.81kD to Mouse NP_659033.1). \nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A0220-02

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Hongo S, Watanabe T, Arita S, Kanome T, Kageyama H, Shioda S, Miyazaki A, Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages. American journal of physiology. Endocrinology and metabolism 2009 Aug 297 (2): E474-82.

Additional Info

Recognizes human ACAT1. Species crossreactivity: mouse, rat. Species sequence homology: canine, bovine.

Alternative Names

acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, ACAT, acetyl-Coenzyme A acetyltransferase 1, ACAT1